Beta-Actin Antibody
Mouse Monoclonal Antibody (Mab)
OVERVIEW
AM1829b staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
OVERVIEW
Overlay histogram showing A431 cells stained with AM1829b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM1829b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed..
OVERVIEW
All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
OVERVIEW
All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
OVERVIEW
Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
OVERVIEW
Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
OVERVIEW
Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35μg/lane). ACTB (arrow) was detected using the purified Mab.
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Product info
Application IHC-P, IF, WB, FC, E
Primary Accession P60709
Reactivity Human, Mouse, Rat
Predlicted Xenopus, Bovine, Chicken, Monkey, Pig, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1,Igkκ
Clone Names 137CT26.1.1
Calculated MW 41737 Da
Additional info
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB5
Target/Specificity This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
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