PCNA Antibody (C-term)
Mouse Monoclonal Antibody (Mab)
OVERVIEW
AM2237b staining PCNA in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
OVERVIEW
All lanes : Anti-PCNA Antibody (C-term) at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: L929 whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
OVERVIEW
Immunohistochemical analysis of paraffin-embedded H. lymph section using PCNA Antibody (C-term)(Cat#AM2237B). AM2237B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product info
Application IHC-P, WB, E
Primary Accession P12004
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1157CT1.1.3
Calculated MW 28769 Da
Additional info
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB5
Target/Specificity This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
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