CHO Host Cell Protein ELISA Kit
This kit is used for determining the presence of Chinese Hamster Ovary (CHO) protein impurities in products manufactured by recombinant expression in CHO host cells. The kit is for Research and Manufacturing Use Only.
How does this kit work ?
The CHO assay is a double antibody sandwich ELISA assay. Samples containing CHO HCPs are reacted simultaneously with an affinity purified capture anti-CHO antibody (goat polyclonal) and a horseradish peroxidase (HRP) enzyme labeled anti-CHO antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The plate are washed to remove any unbound reactants. The substrate TMB is then reacted. The amount of hydrolyzed substrate is read on a microplate reader and is directly proportional to the concentration of CHO Host Cell Proteins present.
What is in the ELISA Kit Box?
Component Specification Stored
Anti-CHO antibody coated plate 12x8 well 2~8℃
CHO HCPs Standards
(0, 1, 3, 10, 40 and 100ng/mL)
0.5mL/vial 2~8℃
Anti-CHO antibody conjugated HRP 10μL/vial 2~8℃
10 × Wash solution 1x60mL 2~8℃
Substrate A 1x10mL 2~8℃
Substrate B 1x10mL 2~8℃
Stop Solution 1x10mL 2~8℃
Handbook 1 sheet
User Handbook
What are the advantages of the ELISA Kit ?
High Purity
Purity > 95%
Purity > 95%
High Coverage
2D-WB coverage rate: > 60%
2D-WB coverage rate: > 60%
High Recovery
The accepted criteria  for recovery rate is 80%-120%.
The accepted criteria
for recovery rate is 80%-120%.
High Sensitivity
Purity > 95%
Purity > 95%
High Coverage
Purity > 95%
37ºC accelerated stability study for pre-coated plate 37ºC accelerated stability study for Standards
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