WuXi will not claim any rights for the result if clients need to apply for patent or other med-chem activities. However, WuXi do need to limit client not to release all the structures to third party at one time to avoid the potential malicious business competition. You can check our description for Permitted Activities.
We will provide you final report after the data analysis. Furthermore, if you purchase the data package we will show you all the enriched compounds with basic information as well as its structure.
We recommend using these three kinds of beads, His-tagged, GST-tagged and anti-FLAG, and other kinds of beads are also acceptable as long as the protein passes the beads capture test mentioned in the protocol.
Normally, the shelf life of DEL Kit is 3 months. Please keep at -80℃.
No target control is indispensable to data analysis. We usually set up one NTC for a kit with one bead type.
There are 14 billion compounds in each tube, and each kit contains 4 same tubes.
It’s not necessary to set all 4 conditions. However, you must set up a condition as No Target Control, which helps to exclude signal from matrix binders.
It is not recommended to do that.
I think it is quite possible.The operation of the Affinity selection is not complicated. Anyone with basic biochemical experiment skill will handle it.
It will take about a whole day (around 6-8 hours) to do a beads capture test and affinity selection.
The post-selection sample should be stored at ultra-low temperatures (-80 ˚C or dry ice) during both storage and shipment.
Yes. The pH of the buffers can be adjusted based on your needs.
Theoretically speaking, the pH can be adjusted as long as the protein can tolerate the condition. But we haven’t tried such low pH yet. So I’m sorry that we don’t have recommend recipe for the low pH buffer.
>10-fold Kd/IC50 value and more than 10-fold for the inhibitor amount to target amount are needed.
It is OK to adjust the recipe of the buffers to suit your needs. In fact, we also added Mg2+ to the buffers in some cases in our full package service. The concentration also has no problem. We haven’t observed any specific binding problem until now.
NGS takes a week. The total workflow is: sample preparation for about a week, NGS for a week, data analysis and writing report for about another week. So from receiving samples to report it will take about 3 weeks or a month.But we don’t guarantee a month I assume.
The molecules in the line-plot are classified into two types: a full length compound or a chemotype. Full length compound is self-explaining and it represents a real molecule; while a chemotype is more abstract. A chemotype does not represent a real molecule. Instead, it provides indication of the performance of specific building blocks. For instance, high enrichment of a chemotype may indicate a possibility of binding activity from a series of molecules that carry those specific building blocks.
Generally speaking, the more the binders, the better the screening result. The average range of enriched binders in one screening is 100-300.
Enrichment= (copy count*total diversity)/total number of reads
Please see the reference.
Kuai, Letian, Thomas O’Keeffe, and Christopher Arico-Muendel. "Randomness in DNA encoded library selection data can be modeled for more reliable enrichment calculation." SLAS DISCOVERY: Advancing Life Sciences R&D 23.5 (2018): 405-416.
Yes, a molecule with higher enrichment level has a higher possibility of being a real binder. However, the binding affinity of the compound needs further confirmation regardless of its enrichment level.
The cost of on-DNA ASMS validation is $750 per compound.
Yes, you can pick up the compound more than 5, and the fee was $2000/cmpd.
$200/Compound (same synthetic route with product)
$3,000/Compound (separate synthetic route with product)